Antibodies for detection of GFP, ZsGreen1, AcGFP1, or EGFP (2023)

FAQs

Do GFP antibodies recognize EGFP? ›

Q: What GFP variants will this antibody recognize? A: Our anti-GFP antibody is a mouse monoclonal raised against the N-terminal of GFP and will recognize native and denatured GFP and its variants, including EGFP, YFP, EYFP, and CFP.

Anti-GFP antibody (ab290) is a highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin.

Green fluorescent protein (GFP) is a 27 kDa monomeric protein, isolated from the jellyfish Aequorea Victoria that auto-catalytically forms a fluorescent pigment. GFP is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression.

The key difference between GFP and EGFP is that the GFP is a wild-type protein incorporated in the molecular cloning of non-mammalian cells while the EGFP is an improved or engineered type of GFP that can be used on mammalian cells.

Flow cytometry and fluorescent microscopy are two conventional tools to detect the GFP signal; flow cytometry is an effective and sensitive technique to quantitatively analyze fluorescent intensity, while fluorescent microscopy can visualize the subcellular location and expression of GFP.

Check that the immunogen is identical to or contained within the region of the protein you are trying to detect. For example, if you are trying to detect a cell surface protein on live cells by FACS, choose an antibody that is raised against an extracellular domain of the protein.

Anti-GFP antibodies are useful reagents for the immunodetection of GFP (green fluorescent protein), which is commonly used as an epitope tag for recombinant proteins in applications such as immunofluorescence, flow cytometry, or western blot.

Identification of GFP-TEMEM184A can also be accomplished using antibodies against GFP. Evaluate both cell samples by identical microscopy techniques.

GFP-tagging is a way of preparing a sample for fluorescence microscopy by using the GFP as a fluorescent protein reporter. This is done by cloning the GFP in frame with the target protein at either the N- or C-terminus of the amino acid chain.

What is GFP tagged protein? ›

The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. The label GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria and is sometimes called avGFP.

Green fluorescent protein is a quantitative reporter of gene expression in individual eukaryotic cells.

GFP allowed the detection, determination of spatial location and enumeration of bacterial cells from diverse environmental samples such as biofilm and water. The gfp as a biomarker was very useful in monitoring of gene expression and protein localisation in bacterial cells, too.

For this type of IFA test, cells are cultured and then placed on a glass slide. Each slide is then incubated with a different concentration of the patient's antibody, which is then washed to remove any unbound proteins.

Although both fluorescent proteins are configured in a β-barrel structure [59], ZsGreen1 exists as a tetramer, whereas GFP (derived from jellyfish) is a monomer that frequently dimerizes for solubility [61]. ...

GFP-like proteins are very diverse, as they can be not only green, but also blue, orange-red, far-red, cyan, and yellow. They also can have dual-color fluorescence (e.g., green and red) or be non-fluorescent.

Enhanced green fluorescent protein (EGFP) is a widely used variant of GFP, which has mutations at two positions: F64L and S65T [9, 10]. EGFP is brighter and matures rapidly at 37°C than wild-type GFP [1, 9].

GFP Chemiluminescent Western Blot Kit allows for the detection of GFP-tagged recombinant proteins present in cell lysates provided by the user. After protein separation by SDS-PAGE and transfer, the membrane is probed with monoclonal Anti-GFP.

When tagged to the protein of interest, the natural fluorescence of GFP provides a molecular label that is both innocuous to the cell and readily visualized by fluorescence light microscopy [5–7].

How is GFP used in the lab? ›

GFP has been recognized as a marker in intact cells for gene expression and protein targeting. In biological studies, it is extensively used as genetically encoded fluorescent markers. This fluorescent marker enables multicolor labeling and is used in the study of interactions between proteins.

A primary antibody binds directly to a particular antigen. In this case, it would be the target protein in your experiment. A secondary antibody binds to the primary antibody instead of binding to the target antigen.

GFP fusions permit analysis of proteins in living cells and offer distinct advantages over conventional immunofluorescence. Among these are lower background, higher resolution, robust dual color colocalization, and avoidance of fixation artifacts.

GFP is amazingly useful for studying living cells, and scientists are making it even more useful. They are engineering GFP molecules that fluoresce different colors. Scientists can now make blue fluorescent proteins, and yellow fluorescent proteins, and a host of others.

Antigen: Green fluorescent protein (GFP) variant Available to For-Profits: Yes Antigen Species: Aequorea victoria Hybridoma Cells Available (Non-Pr...

Immunofluorescence and immunohistochemistry are methods that use antibodies for detection and localization of proteins and other antigens within biological samples. Tissues and other samples are prepared by a process call “fixation” which is usually done directly on microscopy slides.

The antibody screening test performed in a clinical laboratory and/or blood bank is designed to detect the presence of unexpected antibodies, especially alloantibodies in the serum to antigens of the non-ABO blood group system: Duffy, Kell, Kidd, MNS, P, and certain Rh types that are considered clinically significant.

Tests that specifically detected IgG or IgM antibodies were the most accurate and, when testing people from 21 days after first symptoms, they detected 93% of people with COVID-19. Tests gave false positive results for 1% of those without COVID-19.

How to design GFP tagged protein? ›

Solutions of purified GFP look yellow under typical room lights, but when taken outdoors in sunlight, they glow with a bright green color. The protein absorbs ultraviolet light from the sunlight, and then emits it as lower-energy green light.

The GFP gene can be inserted downstream of the promoter of a gene in another organism. RNA polymerase binds to promoter regions to initiate transcription. If the GFP gene is inserted correctly, it can be expressed in organisms other than jellyfish.

One powerful application of genome engineering is the tagging of proteins with fluorescent reporters such as green fluorescent protein (GFP). For this application, the donor DNA consists of a fluorescent reporter–encoding sequence flanked by homology arms matching the sequence around the Cas9-induced cleavage site.

EGFP is a basic (constitutively fluorescent) green fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity.

The enhanced green fluorescent protein (eGFP) is one of the most commonly used marker proteins as its expression can be easily monitored by flow cytometry or confocal microscopy (Bierhuizen et al., 1997).

Pro-GFP is a latent fluorophore activated by proteolysis.

In recent years, several different color forms of GFP have been produced. These are in order from shortest to longest emission spectra: blue, cyan, green, and yellow FP or BFP, CFP, GFP and YFP.

Immunofluorescence assay (IFA) is a standard virologic technique to identify the presence of antibodies by their specific ability to react with viral antigens expressed in infected cells; bound antibodies are visualized by incubation with fluorescently labeled antihuman antibody.

What is the direct fluorescent antibody test for T pallidum? ›

USE OF TEST: The direct fluorescent antibody (DFA) test is performed on lesion exudate to detect the presence of Treponema pallidum, the causative agent of syphilis. SPECIMEN TYPE: Specimen Required: Smear from suspected lesions.

All six of our anti-GFP antibodies are suited for detection of native GFP, GFP variants, and most GFP fusion proteins. The affinity-purified anti-RFP antibody is used to detect native TagRFP and most fusion proteins derived from Entacmaea quadricolor.

Although both GFP and YFP are recognized by the GFP-nanobody, the related fluorescent proteins such as CFP, mFruit series, and other red-shifted proteins are not.

The RFP emission filter permits signals with 575 nm to 640 nm to pass through and the GFP emission filter permits fluorescent signals with wavelengths of 510 nm to 523 nm to pass through. These images confirm that the emission filter blocks unwanted signals efficiently and provide a clean image.

GFP-tagging is a way of preparing a sample for fluorescence microscopy by using the GFP as a fluorescent protein reporter. This is done by cloning the GFP in frame with the target protein at either the N- or C-terminus of the amino acid chain.

Like the parent GFP, YFP is a useful tool in cell and molecular biology because the excitation and emission peaks of YFP are distinguishable from GFP which allows for the study of multiple processes/proteins within the same experiment.

GFP and YFP are two very different fluorescent proteins derived from Aequorea victoria , a jellyfish. Many marine organisms have similar green fluorescent proteins, but GFP refers to a protein which was originally isolated from this particular jellyfish. YFP is a genetic mutant of the GFP.

Concurrent use of GFP and YFP could be highly advantageous in many cellular assays, particularly when using cells of transgenic mice whose cells express fluorescent proteins, because generating mice with alternative fluorescent proteins can take a lot of time and effort.

Although both fluorescent proteins are configured in a β-barrel structure [59], ZsGreen1 exists as a tetramer, whereas GFP (derived from jellyfish) is a monomer that frequently dimerizes for solubility [61]. ...

The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range.

What wavelength is GFP detected at? ›

GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm.

GFP absorbs blue light at 395 nm, with a smaller peak at 475 nm, and emits green light at 508 nm with a quantum yield of 0.72–0.85 (12, 13).